Cell and Molecular Biology: Concepts and Experiments
7th Edition

Cell and Molecular Biology: Concepts and Experiments
Gerald Karp

© 2013

WileyPLUS is a research-based online environment for effective teaching and learning. WileyPLUS builds students’ confidence by taking the guesswork out of studying and providing a clear roadmap; what to do, how to do it, if they did it right. With WileyPLUS students take more initiative so you’ll have a greater impact.


Prepare and Present

  • Course Materials to help you personalise lessons and optimise your time, including:
  • Instructor's Manual and Lecture Outlines
  • Test Bank (Word Document) and Computerised Test Bank
  • Various PowerPointsTM-Lecture Notes, Text Art, Text Table
  • Classroom Response System (Clickers) questions
  • Question Pooling-create question pools based on topic/chapter
  • Question Preview-easily preview a question when creating an assignment by rolling your mouse over it
  • WileyPLUS/Quickstart assignments and presentations for the entire course created by a subject expert
  • Variety of Student Resources including Animations and Videos illustrating key concepts
  • Biochemistry and Molecular Life Sciences Visual Library-includes images, photos and media in a key-term searchable format

Read, Study and Practice

  • Complete online version of the textbook included
  • Relevant, student study tools and learning resources to ensure positive learning outcomes, including:
  • A complete online version of the text (eBook)
  • New Figure in Focus Feature- selected figures include podcasts and assessment questions, highlighting important concepts and processes
  • Clinical Case Studies
  • Flash Cards for interactive review
  • Chapter Overviews and Objectives
  • Self-Quizzing with immediate response feedback
  • Biology Basics-interactive modules on basic concepts for review
  • CellView Tutorial Animations and Video Library with 68 videos
  • Interactive Periodic Table of the Elements
  • Biology Newsfinder -- topical articles in the news, refreshed every 15 minutes


  • Pre-created activities to encourage learning outside of the classroom, including:
  • Question Pooling and Question Previewing.
  • Pre-lecture Quiz questions gauge student's knowledge before class
  • Post-lecture quizzes to test students' mastery after class
  • Select End of chapter questions which may include review questions, problems, and additional exercises
  • Testing capabilities-timed assignments, measuring time on task; disabling feedback; hidden question names, testbank with multitude of questions.
  • Video-based essay questions
  • Video Case questions for review of both continuing cases and end of chapter cases
  • Diagnostic reporting: track student progress by learning objective
  • Clinical Connections and Case questions
  • Extensive questions sets available include Art questions, Human Perspective and Experimental Pathways questions, Analytic and Critical Thinking questions.


  • Study Objective reporting delivers an individualised report on how that student has been doing against each study objective that's been covered within a specific assignment. It enables students to determine where they should focus their time.
  • Instructors can see students' performance on assignments and identify problem areas at a glance.

Topics covered

1 Introduction to the Study of Cell and Molecular Biology

  1. The Discovery of Cells
  2. Basic Properties of Cells
  3. Two Fundamentally Different Classes of Cells
  4. Viruses

2 The Chemical Basis of Life

  1. Covalent Bonds
  2. Noncovalent Bonds
  3. Acids, Bases, and Buffers
  4. The Nature of Biological Molecules
  5. Four Types of Biological Molecules
  6. The Formation of Complex Macromolecular Structures

3 Bioenergetics, Enzymes, and Metabolism

  1. Bioenergetics
  2. Enzymes as Biological Catalysts
  3. Metabolism

4 The Structure and Function of the Plasma Membrane

  1. An Overview of Membrane Functions
  2. A Brief History of Studies on Plasma Membrane Structure
  3. The Chemical Composition of Membranes
  4. The Structure and Functions of Membrane Proteins
  5. Membrane Lipids and Membrane Fluidity
  6. The Dynamic Nature of the Plasma Membrane
  7. The Movement of Substances Across Cell Membranes
  8. Membrane Potentials and Nerve Impulses 

5 Aerobic Respiration and the Mitochondrion

  1. Mitochondrial Structure and Function
  2. Oxidative Metabolism in the Mitochondrion
  3. The Role of Mitochondria in the Formation of ATP
  4. Translocation of Protons and the Establishment of a Proton-Motive Force
  5. The Machinery for ATP Formation
  6. Peroxisomes

6 Photosynthesis and the Chloroplast

  1. Chloroplast Structure and Function
  2. An Overview of Photosynthetic Metabolism
  3. The Absorption of Light
  4. Photosynthetic Units and Reaction Centers
  5. Photophosphorylation
  6. Carbon Dioxide Fixation and the Synthesis of Carbohydrate

7 Interactions Between Cells and Their Environment

  1. The Extracellular Space
  2. Interactions of Cells with Extracellular Materials
  3. Interactions of Cells with Other Cells
  4. Tight Junctions: Sealing The Extracellular Space
  5. Gap Junctions and Plasmodesmata: Mediating Intercellular Communication
  6. Plasmodesmata
  7. Cell Walls

8 Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

  1. An Overview of the Endomembrane System 
  2. A Few Approaches to the Study of Endomembranes
  3. The Endoplasmic Reticulum
  4. The Golgi Complex
  5. Types of Vesicle Transport and Their Functions
  6. Lysosomes
  7. Plant Cell Vacuoles
  8. The Endocytic Pathway: Moving Membrane and Materials into the Cell Interior
  9. Posttranslational Uptake of Proteins by Peroxisomes, Mitochondria, and Chloroplasts

9 The Cytoskeleton and Cell Motility

  1. Overview of the Major Functions of the Cytoskeleton
  2. The Study of the Cytoskeleton
  3. Microtubules
  4. Intermediate Filaments
  5. Microfilaments
  6. Muscle Contractility
  7. Nonmuscle Motility

10 The Nature of the Gene and the Genome

  1. The Concept of a Gene as a Unit of Inheritance
  2. Chromosomes: The Physical Carriers of the Genes
  3. The Chemical Nature of the Gene
  4. The Structure of the Genome
  5. The Stability of the Genome
  6. Sequencing Genomes: The Footprints of Biological Evolution

11 Gene Expression: From Transcription to Translation

  1. The Relationship between Genes, Proteins, and RNAs
  2. An Overview of Transcription in Both Prokaryotic and Eukaryotic Cells
  3. Synthesis and Processing of Eukaryotic Ribosomal and Transfer RNAs
  4. Synthesis and Processing of Eukaryotic Messenger RNAs
  5. Small Regulatory RNAs and RNA Silencing Pathways
  6. Encoding Genetic Information
  7. Decoding the Codons: The Role of Transfer RNAs
  8. Translating Genetic Information

12 Control of Gene Expression

  1. Control of Gene Expression in Bacteria
  2. Control of Gene Expression in Eukaryotes: Structure and Function of the Cell Nucleus
  3. An Overview of Gene Regulation in Eukaryotes
  4. Transcriptional Control
  5. RNA Processing Control
  6. Translational Control
  7. Posttranslational Control: Determining Protein Stability

13 DNA Replication and Repair

  1. DNA Replication
  2. DNA Repair
  3. Between Replication and Repair

14 Cellular Reproduction

  1. The Cell Cycle
  2. M Phase: Mitosis and Cytokinesis
  3. Meiosis

15 Cell Signaling and Signal Transduction: Communication Between Cells

  1. The Basic Elements of Cell Signaling Systems
  2. A Survey of Extracellular Messengers and Their Receptors
  3. G Protein-Coupled Receptors and Their Second Messengers
  4. Protein-Tyrosine Phosphorylation as a Mechanism for Signal Transduction
  5. The Role of Calcium as an Intracellular Messenger
  6. Convergence, Divergence, and Cross-Talk Among Different Signaling Pathways
  7. The Role of NO as an Intercellular Messenger
  8. Apoptosis (Programmed Cell Death)

16 Cancer

Basic Properties of a Cancer Cell

  1. The Causes of Cancer
  2. The Genetics of Cancer
  3. New Strategies for Combating Cancer

17 The Immune Response

  1. An Overview of the Immune Response
  2. The Clonal Selection Theory as It Applies to B Cells
  3. T Lymphocytes: Activation and Mechanism of Action
  4. Selected Topics on the Cellular and Molecular Basis of Immunity

18 Techniques in Cell and Molecular Biology

  1. The Light Microscope
  2. Transmission Electron Microscopy
  3. Scanning Electron and Atomic Force Microscopy
  4. The Use of Radioisotopes
  5. Cell Culture
  6. The Fractionation of a Cell’s Contents by Differential Centrifugation
  7. Isolation, Purification, and Fractionation of Proteins
  8. Determining the Structure of Proteins and Multisubunit Complexes
  9. Fractionation of Nucleic Acids
  10. Nucleic Acid Hybridisation
  11. Chemical Synthesis of DNA
  12. Recombinant DNA Technology
  13. Enzymatic Amplification of DNA by PCR
  14. DNA Sequencing
  15. DNA Libraries
  16. DNA Transfer into Eukaryotic Cells and Mammalian Embryos
  17. Determining Eukaryotic Gene Function by Gene Elimination or Silencing
  18. The Use of Antibodies

Glossary G-1

Additional Readings A-1

Index I-1